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  1. Barna Package
  2. BARNA-54

Sequencing fails with JVM crash related to ZIP file

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    Description

      This happens on the cluster with large data:

      Starting
      Writing parameter file
      Parameters written:
      NB_MOLECULES  250000000
      READ_NUMBER 100000000
      #NB_MOLECULES  250000
      #READ_NUMBER 100000
      READ_LENGTH 76
      ERR_FILE 76
      PAIRED_END  true
      FASTA   true
      
      
      FRAGMENTATION   true
      FRAG_SUBSTRATE  RNA
      #FRAG_EZ_MOTIF
      FRAG_METHOD UR
      
      PCR_DISTRIBUTION    default
      
      FILTERING   YES
      SIZE_SAMPLING   MH
      SIZE_DISTRIBUTION /home/devel/thasso/data/simulator_insert_size_dist.isizes
      
      RTRANSCRIPTION  true
      #RT_MOTIF
      RT_PRIMER   RH
      RT_LOSSLESS true
      REF_FILE_NAME _uncompressed_13235_dm3_flyBaseGene_fromUCSC111123.gtf
      GEN_DIR /scratch/devel/thasso/data/jip/data/genomes/dm3
      PRO_FILE_NAME /scratch/devel/thasso/data/jip/runs/SO-sim3_212/profiler/SO-Simulation.pro
      LIB_FILE_NAME /scratch/devel/thasso/data/jip/runs/SO-sim3_212/lib/lib_split_7/SO-Simulation-8.lib
      SEQ_FILE_NAME SO-Simulation-8.bed
      TMP_DIR /scratch/devel/thasso/data/jip/runs/SO-sim3_212/sequencer/sequencer_split_7/tmpdir
      Starting simulator
      [INFO] I am the Flux Toolbox (v1.0-SNAPSHOT build 1096), nice to meet you!
      
      [INFO] I am collecting information on the run.
              initializing profiler  [DEBUG] Counted 21223 in /scratch/devel/thasso/data/jip/runs/SO-sim3_212/profiler/SO-Simulation.pro
      **********
      
      [INFO] Loading Fragmentation from /scratch/devel/thasso/data/jip/runs/SO-sim3_212/lib/lib_split_7/SO-Simulation-8.lib
      [INFO] Reading error model 76 bases model
      
      [INFO] Checking GTF file
      *[WARN] Unsorted in line 667 - chr/strand chr4 + already read.
      [GTF FILE] The GTF reference file given is not sorted, sorting it right now...
              sorting GTF file  OK (00:00:24)
      [GTF FILE] The Simulator will use /scratch/devel/thasso/data/jip/runs/SO-sim3_212/sequencer/sequencer_split_7/_uncompressed_13235_dm3_flyBaseGene_fromUCSC111123_sorted.gtf
      [GTF FILE] You might want to update your parameters file
      [INFO] you did not ask for expression, I skip it.
      
      [INFO] you did not want me to construct the library, I skip it.
      
      
      [SEQUENCING] getting the reads
              initialize
              zipping ********** 2141523725 lines zipped (3302655675 fragments) (03:10:45)
              sequencing #
      # A fatal error has been detected by the Java Runtime Environment:
      #
      #  SIGSEGV (0xb) at pc=0x00002aaaaad181b0, pid=13298, tid=1092548928
      #
      # JRE version: 6.0_26-b03
      # Java VM: Java HotSpot(TM) 64-Bit Server VM (20.1-b02 mixed mode linux-amd64 compressed oops)
      # Problematic frame:
      # C  [libzip.so+0xb1b0]  __int128+0x60
      #
      # An error report file with more information is saved as:
      # /scratch/devel/thasso/data/jip/runs/SO-sim3_212/sequencer/sequencer_split_7/hs_err_pid13298.log
      #
      # If you would like to submit a bug report, please visit:
      #   http://java.sun.com/webapps/bugreport/crash.jsp
      # The crash happened outside the Java Virtual Machine in native code.
      # See problematic frame for where to report the bug.
      #
      /project/devel/rna_pipeline/usr/bin/flux: line 50: 13298 Aborted                 java -Xmx$FLUX_MEM -DwrapperDir="$dir/bin" $MISC -cp $cp fbi.commons.flux.Flux "$@"
      

      The stacktraces:

      Java frames: (J=compiled Java code, j=interpreted, Vv=VM code)
      j  java.util.zip.ZipFile.getNextEntry(JI)J+0
      j  java.util.zip.ZipFile.access$400(JI)J+2
      j  java.util.zip.ZipFile$2.nextElement()Ljava/util/zip/ZipEntry;+54
      j  java.util.zip.ZipFile$2.nextElement()Ljava/lang/Object;+1
      j  fbi.genome.sequencing.rnaseq.simulation.Sequencer.sequence(Ljava/io/File;Ljava/io/File;)Z+258
      j  fbi.genome.sequencing.rnaseq.simulation.Sequencer.call()Ljava/lang/Void;+83
      j  fbi.genome.sequencing.rnaseq.simulation.SimulationPipeline.call()Ljava/lang/Void;+605
      j  fbi.genome.sequencing.rnaseq.simulation.SimulationPipeline.call()Ljava/lang/Object;+1
      j  fbi.commons.flux.Flux.main([Ljava/lang/String;)V+485
      v  ~StubRoutines::call_stub
      

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            thasso Thasso Griebel
            thasso Thasso Griebel
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            Dates

              Created:
              Updated:
              Resolved: