Details
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Bug
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Status: Resolved
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Major
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Resolution: Fixed
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None
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None
Description
This happens on the cluster with large data:
Starting Writing parameter file Parameters written: NB_MOLECULES 250000000 READ_NUMBER 100000000 #NB_MOLECULES 250000 #READ_NUMBER 100000 READ_LENGTH 76 ERR_FILE 76 PAIRED_END true FASTA true FRAGMENTATION true FRAG_SUBSTRATE RNA #FRAG_EZ_MOTIF FRAG_METHOD UR PCR_DISTRIBUTION default FILTERING YES SIZE_SAMPLING MH SIZE_DISTRIBUTION /home/devel/thasso/data/simulator_insert_size_dist.isizes RTRANSCRIPTION true #RT_MOTIF RT_PRIMER RH RT_LOSSLESS true REF_FILE_NAME _uncompressed_13235_dm3_flyBaseGene_fromUCSC111123.gtf GEN_DIR /scratch/devel/thasso/data/jip/data/genomes/dm3 PRO_FILE_NAME /scratch/devel/thasso/data/jip/runs/SO-sim3_212/profiler/SO-Simulation.pro LIB_FILE_NAME /scratch/devel/thasso/data/jip/runs/SO-sim3_212/lib/lib_split_7/SO-Simulation-8.lib SEQ_FILE_NAME SO-Simulation-8.bed TMP_DIR /scratch/devel/thasso/data/jip/runs/SO-sim3_212/sequencer/sequencer_split_7/tmpdir Starting simulator [INFO] I am the Flux Toolbox (v1.0-SNAPSHOT build 1096), nice to meet you! [INFO] I am collecting information on the run. initializing profiler [DEBUG] Counted 21223 in /scratch/devel/thasso/data/jip/runs/SO-sim3_212/profiler/SO-Simulation.pro ********** [INFO] Loading Fragmentation from /scratch/devel/thasso/data/jip/runs/SO-sim3_212/lib/lib_split_7/SO-Simulation-8.lib [INFO] Reading error model 76 bases model [INFO] Checking GTF file *[WARN] Unsorted in line 667 - chr/strand chr4 + already read. [GTF FILE] The GTF reference file given is not sorted, sorting it right now... sorting GTF file OK (00:00:24) [GTF FILE] The Simulator will use /scratch/devel/thasso/data/jip/runs/SO-sim3_212/sequencer/sequencer_split_7/_uncompressed_13235_dm3_flyBaseGene_fromUCSC111123_sorted.gtf [GTF FILE] You might want to update your parameters file [INFO] you did not ask for expression, I skip it. [INFO] you did not want me to construct the library, I skip it. [SEQUENCING] getting the reads initialize zipping ********** 2141523725 lines zipped (3302655675 fragments) (03:10:45) sequencing # # A fatal error has been detected by the Java Runtime Environment: # # SIGSEGV (0xb) at pc=0x00002aaaaad181b0, pid=13298, tid=1092548928 # # JRE version: 6.0_26-b03 # Java VM: Java HotSpot(TM) 64-Bit Server VM (20.1-b02 mixed mode linux-amd64 compressed oops) # Problematic frame: # C [libzip.so+0xb1b0] __int128+0x60 # # An error report file with more information is saved as: # /scratch/devel/thasso/data/jip/runs/SO-sim3_212/sequencer/sequencer_split_7/hs_err_pid13298.log # # If you would like to submit a bug report, please visit: # http://java.sun.com/webapps/bugreport/crash.jsp # The crash happened outside the Java Virtual Machine in native code. # See problematic frame for where to report the bug. # /project/devel/rna_pipeline/usr/bin/flux: line 50: 13298 Aborted java -Xmx$FLUX_MEM -DwrapperDir="$dir/bin" $MISC -cp $cp fbi.commons.flux.Flux "$@"
The stacktraces:
Java frames: (J=compiled Java code, j=interpreted, Vv=VM code) j java.util.zip.ZipFile.getNextEntry(JI)J+0 j java.util.zip.ZipFile.access$400(JI)J+2 j java.util.zip.ZipFile$2.nextElement()Ljava/util/zip/ZipEntry;+54 j java.util.zip.ZipFile$2.nextElement()Ljava/lang/Object;+1 j fbi.genome.sequencing.rnaseq.simulation.Sequencer.sequence(Ljava/io/File;Ljava/io/File;)Z+258 j fbi.genome.sequencing.rnaseq.simulation.Sequencer.call()Ljava/lang/Void;+83 j fbi.genome.sequencing.rnaseq.simulation.SimulationPipeline.call()Ljava/lang/Void;+605 j fbi.genome.sequencing.rnaseq.simulation.SimulationPipeline.call()Ljava/lang/Object;+1 j fbi.commons.flux.Flux.main([Ljava/lang/String;)V+485 v ~StubRoutines::call_stub