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  1. Barna Package
  2. BARNA-397

Differential Expression on Flux capacitor output

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    • Story
    • Status: Open
    • Major
    • Resolution: Unresolved
    • Capacitor 1.2.2 (API 1.19)
    • None
    • Capacitor
    • None

    Description

      Dear Team,

      For Differential expression, we are using tweeDEseq method which will take read counts as input and noramlize counts using TMM method and perform pair wise comparisons.
      As RPKM values already already normalized counts, can I consider "reads" section from flux-capacitor results as input to tweeDEseq?

      here is the few lines of output from flux-capacitor

      18 ENSEMBL transcript 158483 213739 . + . transcript_id "ENST00000261601.5"; locus_id "18:158483-213739W"; gene_id "ENSG00000101557.8"; reads 4644.136719; length 4156; RPKM 2497.995850
      18 ENSEMBL transcript 158483 213739 . + . transcript_id "ENST00000383589.1"; locus_id "18:158483-213739W"; gene_id "ENSG00000101557.8"; reads 2171.213379; length 4051; RPKM 1198.126099
      18 ENSEMBL transcript 158536 211350 . + . transcript_id "ENST00000400266.3"; locus_id "18:158483-213739W"; gene_id "ENSG00000101557.8"; reads 72.650101; length 1681; RPKM 96.611931

      Please let me know your suggestions.

      Thanks in Advance
      Fazulur Rehaman

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            micha Micha Sammeth
            Fazulur Fazulur Rehaman
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              Created:
              Updated: