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  1. Barna Package
  2. BARNA-301

NullPointerException while iterating loci in BAM files

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Details

    • Bug
    • Status: Resolved
    • Major
    • Resolution: Fixed
    • Capacitor 1.2.3 (API 1.20)
    • Capacitor 1.3 (API 1.23)
    • Capacitor
    • None

    Description

      Reported by David DeLuca

      [ERROR] Error while iterating loci:
      java.lang.NullPointerException
              at barna.flux.capacitor.reconstruction.FluxCapacitor$LocusSolver.learn(FluxCapacitor.java:857)
              at barna.flux.capacitor.reconstruction.FluxCapacitor$LocusSolver.run(FluxCapacitor.java:242)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.solve(FluxCapacitor.java:2175)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.explore(FluxCapacitor.java:2707)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.getProfile(FluxCapacitor.java:2223)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:1757)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:76)
              at barna.commons.launcher.Flux.main(Flux.java:197)
      java.lang.RuntimeException: java.lang.NullPointerException
              at barna.flux.capacitor.reconstruction.FluxCapacitor.explore(FluxCapacitor.java:2760)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.getProfile(FluxCapacitor.java:2223)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:1757)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:76)
              at barna.commons.launcher.Flux.main(Flux.java:197)
      Caused by: java.lang.NullPointerException
              at barna.flux.capacitor.reconstruction.FluxCapacitor$LocusSolver.learn(FluxCapacitor.java:857)
              at barna.flux.capacitor.reconstruction.FluxCapacitor$LocusSolver.run(FluxCapacitor.java:242)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.solve(FluxCapacitor.java:2175)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.explore(FluxCapacitor.java:2707)
              ... 4 more
      [FATAL] Error occured during scanning
              java.lang.NullPointerException
      
      [COUNT] Counting reads to the following elements: splice junctions, all-intronic regions.
      
      [SOLVE] Deconvolving reads of overlapping transcripts.
              decomposing  [WARN] skipped line 1      HAVANA  gene    317811  328455  .       +       .       transcript_id "ENSG00000240876.1"; havana_gene "OTTHUMG00000156972.1"; level "2"; transcript_name "RP4-669L17.11"; gene_name "RP4-669L17.11"; transcript_status "NOVEL"; gene_id "ENSG00000240876.1"; gene_type "processed_transcript"; transcript_type "processed_transcript"; gene_status "NOVEL";
      [WARN] Error in read ID: could not parse read identifier D0KTLACXX120207:4:1301:12261:69282
      [ERROR] Error while iterating loci:
      java.lang.NullPointerException
              at barna.io.sam.SAMMappingIterator.getMates(SAMMappingIterator.java:101)
              at barna.flux.capacitor.graph.AnnotationMapper.map(AnnotationMapper.java:373)
              at barna.flux.capacitor.reconstruction.FluxCapacitor$LocusSolver.run(FluxCapacitor.java:219)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.solve(FluxCapacitor.java:2175)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.explore(FluxCapacitor.java:2709)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:1763)
              at barna.flux.capacitor.reconstruction.FluxCapacitor.call(FluxCapacitor.java:76)
              at barna.commons.launcher.Flux.main(Flux.java:197)
      
      
      [ERROR] java.lang.NullPointerException
      

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            emilio Emilio Palumbo
            emilio Emilio Palumbo
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              Created:
              Updated:
              Resolved: