Details
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Bug
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Status: Resolved
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Major
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Resolution: Fixed
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Simulator 1.2 (API 1.17)
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None
Description
The 3rd column in the .lib file provides the number of additional molecules produced by PCR amplification, or "0" if the step has been de-activated.
However, when switching off fragmentation and RT, .lib files without this column are generated. In such cases the sequencing module crashes with the output
[SEQUENCING] getting the reads
Initializing Fragment Index
Indexing *
[ERROR] Field 3 not found