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  1. Barna Package
  2. BARNA-390

How is GC distribution used exactly

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    • Bug
    • Status: Open
    • Major
    • Resolution: Unresolved
    • None
    • None
    • Simulator
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    Description

      hi,

      I have a very simple question: PCR amplification is simulated with rounds of amplification which involve binomial sampling and an associated probability p in [0,1].

      I'm interested in using the GC distribution, but how exactly is the density related to the amplification efficiency?

      For example phi(0.5; mean=0.5, SD=0.1) = 3.989423 > 1 which cannot be a probability.

      Is the density cropped at 1? Scaled such that max(phi) = 1?

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            thasso Thasso Griebel
            mikelove Mike Love
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              Created:
              Updated: