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  1. Barna Package
  2. BARNA-275

Paired-end reads in BAM files

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Details

    • Story
    • Status: Resolved
    • Major
    • Resolution: Fixed
    • Capacitor 1.2.3 (API 1.20)
    • Capacitor 1.3 (API 1.23)
    • Capacitor
    • None

    Description

      Hi,

      I was trying to run flux-capacitor with a BAM file with paired-reads and I get an error saying that "Annotation mapping PAIRED requires a paired-end read descriptor!". Then I ran flux-capacitor again with the --read-descriptor=PAIRED option and I got an error saying that I cannot use the read-descriptor option with BAM files:
      [ERROR] You cannot specify the read descriptor when using BAM input files.

      Is this a bug or am I using flux-capacitor with the wrong options?

      Thanks,

      Nuno Fonseca

      flux-capacitor --force --annotation tests/data/reference/homo_sapiens/chr19.gtf -i test4/tophat1/PE1.pe.hits.bam --annotation-mapping PAIRED --threads 1 -p test4/tophat1/PE1.pe.hits.bam.par -o test4/tophat1/flux_cap/PE1.flux_cap.trans.tsv.gtf
      Flux-Capacitor v1.2.3-20121129021848 (Flux Library: 1.20-20121129021848)

      [PRE-CHECK] I am checking availability of the required lpsolve JNI libs.
      [PRE-CHECK] * successfully loaded lpsolve JNI (version 5.5,release 0,build 14)

      [ERROR] Annotation mapping PAIRED requires a paired-end read descriptor!

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            thasso Thasso Griebel
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              Created:
              Updated:
              Resolved: