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  1. Barna Package
  2. BARNA-271

Simulated sequencing cannot handle .lib files without amplification column

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Details

    • Bug
    • Status: Resolved
    • Major
    • Resolution: Fixed
    • Simulator 1.2 (API 1.17)
    • Simulator
    • None

    Description

      The 3rd column in the .lib file provides the number of additional molecules produced by PCR amplification, or "0" if the step has been de-activated.

      However, when switching off fragmentation and RT, .lib files without this column are generated. In such cases the sequencing module crashes with the output

      [SEQUENCING] getting the reads
      Initializing Fragment Index
      Indexing *

      [ERROR] Field 3 not found

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          People

            emilio Emilio Palumbo
            micha Micha Sammeth
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            Dates

              Created:
              Updated:
              Resolved: